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Calculate the pI of glycine using the given values:
Calculate the pI of glycine using the given values: Glycine: COOH⁻ NH₃⁺ pKa₁ = 2.72, pKa₂ = 9.60
a) pI = (pKa₁ + pKa₂) / 2
b) pI = pKa₁ + pKa₂
c) pI = (pKa₁ * pKa₂) / 2
d) pI = sqrt(pKa₁ * pKa₂)
Final answer:The isoelectric point (pI) of glycine is calculated by taking the average of its two pKa values, which is (pKa₁ + pKa₂) / 2, resulting in a pI of 6.16.Explanation:To calculate the pI (isoelectric point) of glycine, you need to use the pKa values of the compound's acidic and basic groups. Glycine has two such groups: the carboxyl group (COOH⁻) with a pKa₁ of 2.72, and the amino group (NH₃⁺) with a pKa₂ of 9.60. The formula for calculating the pI of a compound like glycine, which has no ionizable side chain, is to take the average of the pKa values of the carboxyl and amino groups, therefore, the correct equation is pI = (pKa₁ + pKa₂) / 2. Using this formula, the pI of glycine is calculated as follows:pI = (2.72 + 9.60) / 2 = 6.16The pI is the pH at which the molecule has no net charge, and this is particularly important in understanding its behavior in different pH environments....